Biomolecular membrane protein crystallization
نویسندگان
چکیده
منابع مشابه
Crystallization of Membrane Protein Nanoparticles
The self-assembly of membrane proteins from solution into two-dimensional crystals is studied numerically using a continuum model proposed by Talanquer and Oxtoby (J. Chem. Phys. 109, 223 (1998)). Although the mean field theory presented here is only qualitatively accurate in two dimensions, we obtain the main features of the crystal nucleation process, such as the nucleation free energy barrie...
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Membrane protein structural biology is a frontier area of modern biomedical research. Twenty to thirty-five percent of the proteins encoded by an organism's genome are integral membrane proteins. Integral membrane proteins, such as channels, transporters, and receptors, are critical components of many fundamental biological processes. Also, many integral membrane proteins are important in biome...
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Crystallization is a major bottleneck to obtaining x-ray structures of membrane proteins. By applying an established crystallization protocol for the lactose permease (LacY) of Escherichia coli, a systematic study of the effect of phospholipids (PL) on crystallization of LacY was undertaken. We observe three different crystal forms that diffract to increasingly better resolution in a manner tha...
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When starting a protein-crystallization project, scientists are faced with several unknowns. Amongst them are these questions: (i) is the purity of the starting material sufficient? and (ii) which type of crystallization experiment is the most promising to conduct? The difficulty in purifying active membrane-protein samples for crystallization trials and the high costs associated with producing...
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The Ca2+-ATPase SERCA1a (sarcoplasmic-endoplasmic reticulum Ca2+-ATPase isoform 1a) from rabbit has been overexpressed in Saccharomyces cerevisiae. This membrane protein was purified by avidin agarose affinity chromatography based on natural biotinylation in the expression host, followed by HPLC gel filtration. Both the functional and structural properties of the overexpressed protein validate ...
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ژورنال
عنوان ژورنال: Philosophical Magazine
سال: 2012
ISSN: 1478-6435,1478-6443
DOI: 10.1080/14786435.2012.670734